Biotin, a water-soluble vitamin belonging to the B-complex family, plays a crucial role in supporting healthy hair, skin, and nails. It aids in the differentiation of the epidermis and boosts the production of keratins, which are essential for maintaining the structural integrity of the skin, hair, and nails. Recent clinical studies have suggested that biotin may also influence the cellular arrangement of the nail bed, resulting in strengthened keratin structures, reduced splitting, and increased thickness. Biotin acts as an important co-factor for numerous metabolic carboxylation reactions, such as those involved in carbohydrate, fat, and protein metabolism. Additionally, recent research has revealed that biotin is involved in maintaining healthy genetic expression of many enzymes for which it is a cofactor. For instance, it may promote transcription and translation of glucokinase, an enzyme critical for carbohydrate metabolism in the liver and pancreas.